Oak wood provides suitable nutrients for long-term continuous pesticides removal by Trametes versicolor in a pilot plant trickle bed reactor

In the Catalan area (Spain), diuron and bentazon are extensively used to control weeds therefore abundant in agricultural drainage. White-rot fungi have a high potential for removing these contaminants because of potent nonsubstrate-specific lignin-degrading enzyme system. Unfortunately, bacterial contamination hinders practical application fungal treatment. this study, trickle bed reactor (TBR) containing Trametes versicolor immobilized on oak wood was treat spiked nonsterile wastewater. The TBR operated 186 days continuous mode at different recirculation rates. exhibited stable promising performance, with an average removal up 80% 67%, respectively, steady state. lignocellulosic carrier only carbon resource biomass throughout entire operation period effectively suppressed growth. Adsorption also important contribution pesticide removal. constructed system persistent activity robustness adverse impacts regarding clogging, based pesticides removal, laccase activity, ergosterol quantity scanning electron microscopy analysis. This study demonstrates concept long-term treatment paves way full-scale application.